Valorization of waste by-products of rose oil production as feedstuff phytonutrients.
The aim of this study was to evaluate possibility for valorization of the dry pressed distilled rose petals (DDRP) as a feedstuff phytonutrients in animal husbandry. In connection with the chemical composition and radical scavenging activity of polyphenol complex in rose waste products were studied. The polyphenol composition in dry rose petals, DDRP and waste water after distillation was identified and quantified. The chromatographic analysis was conducted by HPLC-PDA and LC-MS. The experiments were conducted with bird, monogastric and ruminants representatives. A total of 40 one days old chicks from the hybrid combination Ross 308 were distributed into two groups each containing of 20 birds fed for 49 days. The control group C fed with basal diet vs. experimental group R fed with the same fodder plus 40 mg DDRP/kg/d. A total of 72 Danube White 155 days old fattening pigs (both sexes - 36 male and 36 female) of 72.500 ± 1.937 kg were distributed into three groups (one control C and two experimental R1 and R2 supplemented with 0.255 g DDRP/kg/d or 0.545 g DDRP/kg/d). Each one group (24 piglets) was fed for 45 days. A total of 30 numbers of 65 days old lambs from the Synthetic population of Bulgarian milk sheep breed lambs (both sexes - 15 male and 15 female) with average life weight 20.500 ± 1.039 kg were divided into two groups (control C and experimental R) each one containing of 10 animals fed for 50 days. The experimental group was fed with combined feed for lambs granules and ground alfalfa supplemented either 0.545 g DDRP/kg/d. Animals were assessed for average daily weight gain and feed conversion ratio. Thirteen glycosides of kaempferol, ten glycosides of quercetin, six glycosides of gallic acid and the two flavonol aglycones have been identified in dry rose petals. Those polyphenols possess high antioxidant activity and positively influence the growth performance of pigs. The supplementation of pig's feed with 545 mg DDRP/kg/d increased (p < 0.05) with 6.73% the total feed consumption and with 27.05% the average daily weight gain, and decreases with approximately 16% the feed conversion ratio comparing to control group (C) pigs fattening without DDRP supplementation. The studied concentrations of DDRP were not effective in small ruminants and poultry husbandry.Open preprint
This article is a preprint and has not been peer reviewed